ABSTRACT
Objective To investigate the effects of glutathione protected gold nanoclusters (GSH-Au NCs) on HeLa cytotoxity.Methods Fluorescence intensity were measured on GSH-Au NCs containing medium treated cells using fluorescence spectrophotometer at different time points.GSH-Au NCs uptake by HeLa cells at 1,2,6,12 and 24 h were investigated through fluorescent spectrophotometer.In vivo tumor uptake was also investigated on BALB/c tumor-bearing mice through inductively coupled plasma mass spectrometry (ICP-MS) at 24 h after intraperitoneal injection of 0.2 ml GSH-Au NCs (3 mmol/L) and distilled water (control group) respectively.The cytotoxicity of GSH-Au NCs at different doses (0.003-0.3 mmol/L) was tested at 24 and 48 h using MTT assay after interaction with HeLa cells.Results The uptake efficiency of GSH-Au NCs by HeLa cells kept increasing and reached maximum of 73.13％ at 24 h.The results of tumor-bearing mice indicated that the tumor tissue had higher uptake efficiency after 24 h (320±15) ng/g than that of control group (intraperitoneal injection of distilled water),and the difference was stastically significant (P＜0.05).HeLa cells were treated with different concentrations of GSH-Au NCs for 24 h,and GSHAu NCs had a slight effect on cell viability.With the increase of GSH-Au NCs dose,the inhibition effects on growth of HeLa cells enhanced.The cell activity of HeLa cells treated with 0.3 mmol/L GSH-Au NCs for 24 h reduced to 86％compared with that of control group (the concentration of GSH-Au NCs was 0) (P＜0.05),while there was no significant difference between the survival rate of different concentrations of GSH-Au NCs group and the control group for 48 h.Conclusions GSH-Au NCs have neglectable cytotoxity on HeLa cells even though both in vitro and in vivo uptake are high.GSH-Au NCs are suitable for biomedical application such as imaging,drug loading and targeted drug delivery.
ABSTRACT
Cancer has become the second largest life-threatening disease nowadays.Radiotherapy and chemotherapy are still important treatments for cancer.However, they tend to produce a lot of serious adverse effects including bone damage and bone marrow fat, etc.Based on recent research, the research progress on canonical Wnt pathway and its impact on stromal stem cells differentiation into osteoblasts and adipocytes are reviewed.Radiochemotherapy-induced bone damage and bone marrow fat is closely related to canonical Wnt pathway.In experimental assay and clinical application, Wnt pathway antagonists, such as Dickkopf-1 (DKK-1), sclerostin, and secreted frizzled-related protein 1 (sFRP-1) are used to relieve bone damage.Wnt pathway is expected to become a potential target for the therapy of bone damage and bone marrow fat induced by raidochemotherapy.